Owens

TrimGalore

TrimGalore is a wrapper tool that automates quality and adapter trimming to FastQ files. It also provides functionality to RRBS sequence files.

FastQC

FastQC provides quality control checks of high throughput sequence data that identify areas of the data that may cause problems during further analysis.

Owens SSH key fingerprints

These are the public key fingerprints for Owens (in hexadecimal format):
owens: ssh_host_rsa_key.pub = 18:68:d4:b0:44:a8:e2:74:59:cc:c8:e3:3a:fa:a5:3f
owens: ssh_host_ed25519_key.pub = 1c:3d:f9:99:79:06:ac:6e:3a:4b:26:81:69:1a:ce:83
owens: ssh_host_ecdsa_key.pub = d6:92:d1:b0:eb:bc:18:86:0c:df:c5:48:29:71:24:af

GLOBUS OUTAGE 11AM-3PM DEC 9, 2017

An outage of Globus service is scheduled from 11 a.m. to 3 p.m. Saturday, December 9, 2017 for scheduled upgrades from Globus. Active file transfers will be suspended during this time and they will resume when the Globus service is restored. Users trying to access the service at globus.org will see a maintenance page until the service is restored.

Cufflinks

Cufflinks is a program that analyzes RNA -Seq samples. It assembles aligned RNA-Seq reads into a set of transcripts, then inspects the transcripts to estimate abundances and test for differential expression and regulation in the RNA-Seq reads.

HISAT2

HISAT2 is a graph-based alignment program that maps DNA and RNA sequencing reads to a population of human genomes.

TopHat

TopHat uses Bowtie, a high-throughput short read aligner, to analyze the mapping results for RNA-Seq reads and identify splice junctions.

 

Please note that tophat (and bowtie) cannot run in parallel, that is, on multiple nodes.  Submitting multi-node jobs will only waste resources.  In addition you must explicitly include the '-p' option to use multiple threads on a single node.

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