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On July 12th, 2016 OSC migrated its old GPFS and Lustre filesystems to new Project and Scratch services, respectively. We've moved 1.22 PB of data, and the new capacities are 3.4 PB for Project, and 1.1 PB for Scratch. If you store data on these services, there are a few important details to note.
Ascend, Cardinal, Pitzer
Bowtie2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB.
Ascend, Cardinal, Pitzer
While our Python installations come with many popular packages installed, you may come upon a case in which you need an additional package that is not installed. If the specific package you are looking for is available from anaconda.org (formerly binstar.org), you can easily install it and required dependencies by using the conda package manager.
Cardinal
Linaro HPC tools analyze how HPC software runs. It consists of three applications, Linaro DDT, Linaro Performance Reports and Linaro MAP:
Ascend, Cardinal, Pitzer
Sometimes the best way to get access to a piece of software on the HPC systems is to install it yourself as a "local install". This document will walk you through the OSC-recommended procedure for maintaining local installs in your home directory or project space. The majority of this document describes the process of "manually" building and installing your software.
Ascend, Cardinal, Pitzer
STAR: Spliced Transcripts Alignment to a Reference.
Availability and Restrictions
Versions
The following versions of STAR are available on OSC clusters:
Ascend
Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their associated parameters are supplied on the command line.
Availability and Restrictions
Versions
The following versions of Trimmomatic are available on OSC clusters:
Ascend, Cardinal
SnpEff is a variant annotation and effect prediction tool. It annotates and predicts the effects of variants on genes (such as amino acid changes).
Availability and Restrictions
Versions
The following versions of SnpEff are available on OSC clusters:
Cardinal, Pitzer
The Sequence Read Archive (SRA Toolkit) stores raw sequence data from "next-generation" sequencing technologies including 454, IonTorrent, Illumina, SOLiD, Helicos and Complete Genomics. In addition to raw sequence data, SRA now stores alignment information in the form of read placements on a reference sequence. Use SRA Toolkit tools to directly operate on SRA runs.
