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Pitzer

Cell Ranger is a cell analysis library for generate feature-barcode matrices, perform Analysis for RNA samples. Cell Ranger works in pipelines for it's RNA  sequencing analysis which allows it to: process raw sequencing output, read alignment, generate gene-cell matrices, and can perform downstream analyses such as clustering and gene expression analysis.

Gateway, Owens, Pitzer

Add to a Classroom Project

We provide the instructor (PI of the classroom project) with information on how to get students added to the classroom project. Usually, invites will be sent to new OSC users; existing OSC users will be automatically added to the project if they have an OSC account which matches the email information provided by the PI.

SUG Conference - April 14, 2021

Agenda

Pitzer

Unblur is used to align the frames of movies recorded on an electron microscope to reduce image blurrig due to beam-induced motion. It reads stacks of movies that are stored in MRC/CCP4 format. Unblur generates frame sums that can be used in subsequent image processing steps and optionally applies an exposure-dependent filter to maximize the signal at all resolutions in the frame averages. Movie frame sums can also be calculated using Summovie, which uses the alignment resuls from a prior run of Unblur.

Pitzer

OpenCV is an open-source library that includes several hundreds of computer vision algorithms.

In December 2021 OSC updated its firewall to enhance security. As a result, SSH sessions are being closed more quickly than they used to be. It is very easy to modify your SSH options in the client you use to connect to OSC to keep your connection open.

In ~/.ssh/config (use the command touch ~/.ssh/config to create it if there is no exisitng one), you can set 3 options:

Pitzer

MotionCor2 uses multi-GPU acceleration to correct anisotropic cryo-electron microscopy images at the single pixel level across the whole frame, making it suitable for single particle and tomographic images. Iterative, patch-based motion detection is combined with spatial and temporal constraints and dose weighting.

Availability and Restrictions

Versions

The following versions are available on OSC clusters:

Gateway

MyOSC now offers the ability for PIs to view the billing statements of current un-billed usage for their projects.

Login to my.osc.edu and navigate to Project -> Billing Statements.

There are two sections:

Current usage

The current usage section provides information usage charges up to the current day.

Keep protected data in proper locations

Protected data must be stored in predetermined locations. The only locations at OSC to store protected data are /fs/ess/<project-code> and /fs/ess/scratch/<project-code> directories.

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